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Glyphosate Testing: Test Yourself

Glyphosate Testing: Test Yourself

The Detox Project enables you to find out what man-made chemicals are in your body and in your food:

  • 2015 – Test your urine and water for glyphosate (The World’s most used herbicide)
  • 2015 – Test your food for glyphosate
  • 2016 – Test your urine, water and food for 150+ man-made chemicals

Due to the overwhelming interest in the only validated LC/MS/MS glyphosate testing for the public in the World, we have had to temporarily stop the urine and water testing program until we start working with a much larger lab in 2016.

If you are interested in having your urine or water tested enter your e-mail here and we will contact you when the project starts in Summer 2016:


The Detox Project will give you an accurate report on the levels of glyphosate in your urine and water in parts per billion (PPBs) and also gives you information about the results of independent peer-reviewed lab studies on rats and other animals that have been completed on glyphosate and glyphosate -based herbicides.

Disclaimer: The results from the Detox Project glyphosate testing are not to be used for diagnosis, prevention, or treatment of disease or impairment. The results are also not to be used in any way for health assessment. The Detox Project is not suggesting in any way that any health condition you may have will improve if you manage to lower the glyphosate level in your body.

FAQ: Glyphosate Testing (click to read)

Why has this validated glyphosate testing been developed?

Glyphosate is the most widely used herbicide in the U.S. and in the world and one of the most widely used chemicals in our environment. So The Detox Project believes that validated glyphosate testing for the general public is important.

There have only been a very few small-scale studies done to measure the levels of glyphosate in human fluids like breast milk and urine. The testing that has been done so far has either not been made available to the general public or has not used methods that global regulators (such as the Environmental Protection Agency – EPA) will accept.

The validated glyphosate testing method developed thanks to The Detox Project’s sponsorship is one of the first ever environmental chemical tests to be made available to the general public.

The testing method has very low minimum detection levels, which means that the public will be given an accurate and clear idea as to the level of glyphosate found in their body fluids and in their water.

What method is being used for this glyphosate testing?

Glyphosate (N-(phosphomethyl)glycine ) is directly analyzed using liquid chromatography- tandem mass spectrometry (LC-MS/MS). Water and urine samples are prepared for analysis by solid phase extraction using an ion exchange column. Extracted samples are injected to the LC-MS/MS and the analyte is separated using an Obelisc N column (SIELC Technologies, Prospect Heights, IL) through isocratic elution. Ionization of glyphosate is achieved using an electrospray ionization source operated in negative polarity. The analyte is detected by multiple reaction monitoring using a 13C-labelled glyphosate as internal standard. Quantification of the analyte is done by isotope dilution method using an eight-point calibration curve.

The assay has a limit of quantification of 0.5 ng/mL. The intra- and inter-day precision observed are 6-15% in concentrations that range 0.5-80 ng/mL. Recoveries for glyphosate range 70-80% at concentrations within the assay’s linear dynamic range.

How does this validated glyphosate testing improve on other publicly available glyphosate testing?

Previously available glyphosate testing for the general public is based on the ELISA method. This method is not accepted by government regulators.

LC/MS/MS (Liquid chromatography-tandem mass spectrometry) testing methods are the only responsible methods that should be used in testing urine, water or food for glyphosate and AMPA residues. The Detox Project only supports labs that use these methods.

ELISA testing methods for glyphosate and its metabolite AMPA should not be used as they can produce a high level of false positive and false negative results – leading to possibly inaccurate results. ELISA is a screening tool and as such is not designed to provide accurate levels.

Are there ‘safe’ levels of glyphosate?

Read more detail about ‘How safe are “safe” levels of glyphosate?’ here

How am I helping future generations by taking this test?

Your reaction to the testing results could force political change on the issues surrounding glyphosate and environmental man-made chemicals, thus helping to protect public health now and in the future.

Glyphosate has also been possibly linked to many different diseases in correlation and independent peer-reviewed lab studies. To enable independent scientists to design lab-based experiments that give a clear indication of what real-life health effects glyphosate is having on the human population, including on the health of future generations, they need scientifically valid and statistically significant data on what levels of glyphosate are found in the human population and our food.

By taking this test you will help the independent scientific community to bring clarity to the real damage, if any, being done by glyphosate.

How can I detox from glyphosate?

University research has demonstrated that certified organic diets significantly lower children’s dietary exposure to organophosphorus pesticides. Download the research here.

If your first glyphosate test results show significant glyphosate levels in your body, then, based on the above study, eating a certified organic diet (with only filtered water) for 3 weeks could potentially detox the glyphosate from your body. In other words, your second glyphosate test taken after your organic diet will show either a detox of none, some, or all of the glyphosate in your body.

How can glyphosate be phased out of our food and environment?


For full information on The Detox Project Solutions to phase out glyphosate and to support sustainable agriculture click here.

If you have already registered and paid for the glyphosate testing please find the testing instructions here:

Testing Instructions Here (Web-Page)

Download the Testing Instructions Here (PDF)

If you have already paid and registered for the testing, sent in your samples, received your results or have any general questions about The Detox Project please contact